Reproduction of tumor microenvironment in vivo using the conventional 2- or 3-dimensional culture method is difficult because of the lack of extracellular matrix (ECM) and intercellular interaction. In this study, we tried to establish a 3-dimensional tumor environment using spheroids of oral cancer-derived cells. The Interleukin-6 (IL-6) expression level was high in the spheroids on measurement using the real-time RT-PCR method showing a difference from that in single cells. A representative inflammatory cytokine, IL-6, increases the activity of many types of cancer-derived cells, being involved in tumor formation and metastasis, and similar findings were noted in HSC-4 cells derived from human squamous cell carcinoma of the tongue, for which analysis of phenomena in the cellular interface in 3-dimensionally formed masses is expected. It is highly possible that this method is applicable for studies on the carcinogenicity of nanomaterials represented by asbestos.
Key words: spheroid, IL-6, HSC-4 cells
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