Nano Biomedicine

Effects of ES-D3 Cells Exposed to Multi-walled Carbon Nanotubes on Cell Differentiation by Three-dimensional Culture with Tilapia Scale Collagen

Tsubasa SHIRAI1, Mariko NAKAI1, Tadashi YOKOYAMA1, Mari AKIYAMA1, Yoshiya HASHIMOTO1, Koichi IMAI1, Masayuki ZENNYU1, Takamitsu YOSHIDA1, Hideo SHIMIZU2,3, and Shosuke MORITA2

1Department of Biomaterials, 2First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan, 3Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan

Nano Biomed 2018; 10(1): 9-14, (Jun 30, Nano Biomedicine)

Scaffold selection is critical for regenerative medicine. Mammalian (porcine) collagen has been extensively studied as a scaffold. However, collagens derived from mammals close to humans may pose problems associated with viruses contained in scaffolds upon tissue and organ transplantation into humans. Therefore, fish scale collagen from tilapia, which inhabits tropical and subtropical regions, may be more suitable than porcine collagen as a scaffold for regenerative medicine. Tilapia collagen can be utilized in a biological safety test on biomaterials for regenerative medicine. No report has been published on nanomaterials with tilapia collagen. Therefore, we investi-gated cell differentiation using tilapia collagen in a nanomaterial insoluble in body fluids. Specifically, the differentiation of ES-D3 cells exposed to multi-walled carbon nanotubes (MWCNTs) was examined in two-dimensional culture with tilapia collagen. As a result, ES-D3 cells were differentiated with tilapia collagen in the same manner as porcine collagen. MWCNTs had significant effects on the differentiation of ES-D3 cells.

Key words: ES-D3 cells, MWCNTs, three-dimensional culture, tilapia, collagen

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