Nano Biomedicine
ORIGINAL ARTICLE
 Effects of Nano-hydroxyapatite Content on Bone Formation in Nano-hydroxyapatite-collagen Composite Membrane with BMP-2 Application

 Taichi TENKUMO1, Tsutomu SUGAYA1, Masamitsu KAWANAMI1, and Fumio WATARI2

 1Department of Periodontology and Endodontology Division of Oral Health Scinece, Hokkaidou University Graduate School of Dental Medicine. Sapporo, Japan
2Department of Biomedical, Dental Materials & Engineering, Division of Oral Health Scinece, Hokkaidou University Graduate School of Dental Medicine. Sapporo, Japan
Nano Biomedicine 2009;1(2): 159-166, (Dec 30)
Synopsis
The effects of calcium and phosphate contents on bone formation activity by BMP-2 with nano-hydroxyapatite-collagen composites (nHACs) were evaluated. Nanosize crystallites with different concentrations of hydroxyapatite were successfully fabricated by changing the mixing ratio of calcium solution and phosphate-containing neutralization buffer. Composites were implanted into the subcutaneous tissue in the Wistar male rats with BMP-2 for 2 and 4 weeks. nHAC was degraded and inflammation in the surrounding area decreased with time.
Ectopic bone formation was observed around nHACs with higher contents of nano-hydroxyapatite in BMP-2 application at 2 weeks, but was not observed with lower contents at 4 weeks. These results suggest that nHAC with a higher content of nano-hydroxyapatite is suitable as a carrier for BMP-2.

Key words: nanomaterials; hydroxyapatite; BMP-2; bone formation; in vivo

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