Synopsis
Histological investigations of the nanohydroxyapatite-collagen composite(nHAC) foil in tissue were carried out to evaluate its possible suitability as a BMP-2 carrier for regeneration of periodontal tissue. The nanohydroxyapatite-collagen composite and its thin foil could be successfully fabricated with the crosslinking agent of either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride(EDCjor ascorbic acid / copper chloride (AA/CC). They were implanted into the subcutaneous tissue and tight muscle with and without BMP-2 for 1 and 2 weeks after surgery. nHAC was degraded and the inflammation in the surrounding area was decreased with time. nHAC(AA/CC) was degraded earlier and showed a better biocompatibility than nHAC(EDC). In the case of BMP-2 application, the different crosslinking agent showed an inverse effect. New bone formation was observed only in muscle for EDC and only in subcutaneous tissue for AA/CC. The Present results suggested that the nHAC is suitable as the carrier of BMP-2 with either the crosslinking agent of EDC or ascorbic acid / copper chloride, and the suitable crosslinking agent should be selected depending on desired conditions.
Key words: nanomaterials, hydroxyapatite,BMP-2, in vivo
