Nano Biomedicine
An Attempt to Study of the Embryotoxicity by the Diamond Particles of Dental Diamond Points with the Embryonic Stem Cell Test

Koichi IMAI1, Fumio WATARI2, Tsukasa AKASAKA2, Kazuhiko SUESE3 ,4, Fumiya OGAWA5, Hirofumi SAWAI6, and Hiromasa TAKASHIMA7

1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
3Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
4Department of Esthetic Dentistry, Osaka Dental University, Osaka, Japan
5Department of Postgraduate Clinical Training, Osaka Dental University, Osaka, Japan
6Department of Internal Medicine, Osaka Dental University, Osaka, Japan
7Ina Research Inc., Nagano, Japan

Nano Biomedicine 2013;5(2): 104-108, (Dec 30)

Diamond points are used to cut or grind teeth and prostheses in clinical practice. The embryotoxicity of scattered abrasive diamond grains was investigated in patients and practitioners by the Embryonic Stem Cell Test (EST) method. The risks of embryotoxicity should be considered in patients, practitioners, and pregnant and possibly pregnant women. Commercial diamond particles and diamond particles detached by grinding a diamond point were used. Commercial diamond particles slightly lowered ID50 and IC50 values. Diamond particles detached from a diamond point markedly lowered ID50 and IC50 values. These results may be explained by the contaminating plating components of diamond points. In both experiments, ID50 values were higher than IC50 values, demonstrating the low-level effects of diamond particles on ES-D3 cell differentiation.

Key words: dental diamond particle, embryotoxicity, EST

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