Diamond points are used to cut or grind teeth and prostheses in clinical practice. The embryotoxicity of scattered abrasive diamond grains was investigated in patients and practitioners by the Embryonic Stem Cell Test (EST) method. The risks of embryotoxicity should be considered in patients, practitioners, and pregnant and possibly pregnant women. Commercial diamond particles and diamond particles detached by grinding a diamond point were used. Commercial diamond particles slightly lowered ID50 and IC50 values. Diamond particles detached from a diamond point markedly lowered ID50 and IC50 values. These results may be explained by the contaminating plating components of diamond points. In both experiments, ID50 values were higher than IC50 values, demonstrating the low-level effects of diamond particles on ES-D3 cell differentiation.
Key words: dental diamond particle, embryotoxicity, EST